The work in my laboratory is aimed at elucidating the input/output properties of cortical pyramidal neurons and the structure and function of dendritic spines and their role in information processing and plasticity of cortical circuits in health and disease. My laboratory focuses on understanding how layer 5 pyramidal neurons [the main output neuron of the neocortex], and the circuitry they reside in, enables sensory processing. We are extending these questions to neurological disorders, such as Fragile X syndrome, to uncover neuronal and circuit elements that are affected by these diseases. We are taking a multifaceted approach to answering these questions, using optical, electrophysiological, structural, genetic, and molecular biology tools, including a state-of-the-art custom-built in vivo and in vitro 2P setup with holographic stimulation capabilities.